Core Facility for Medical Bioanalytics

Core Facility für Medizinische Bioanalytik

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Services & Instrumentation

Metabolomics

Instrumentation

LC systems Ion sources Mass spectrometers
Eksigent EKSPERT microLC 200 ESI ABSciex QTRAP 6500
ExionLC UHPLC ESI ABSciex Triple Quad 4500
Waters Acquity UPLC ABSciex SelexIon
(ion mobility)		 Thermo Scientific LTQ Orbitrap XL
  APCI  

Services

  Analytes
Method development for targeted small molecule analysis On demand and dependent on instrument availability
Biocrates AbsoluteIDQ® p180 Kit Amino acids (Q), biogenic amines (Q), acetylcarnitines (SQ), sum of hexoses (SQ), sphingomyelins (SQ), phosphatidylcholines (SQ)
Chromsystems Kit Amino acids (SQ) + acetylcarnithines (SQ)
Biocrates Bile Acid Kit human and murine bile acids (Q)
Lipidomics screen 300-400 lipid species (SQ)
Sphingolipids (under development) sulfatides (Q), SM (Q), ceramide trihexosides (Q)
  • Q – quantitative
  • SQ – semi-quantitative

Proteomics

Instrumentation

LC systems Instruments
3x Dionex Ultimate 3000 RCLCnano Thermo Scientific Orbitrap Fusion
Eksigent EKSPERT microLC 200 Thermo Scientific QExactive Plus

 

 Thermo Scientific LTQ Orbitrap Velos

 

 ABSciex QTRAP 6500

Services

Qualitative Proteomics Quantitative Proteomics Protein complex analysis
Shotgun proteomics SILAC Affinity-purification mass spectrometry (AP-MS)
Targeted (SRM, PRM) Label-free quantification quantitative AP-MS
  Targeted (SRM, PRM) Complex comparison (i.e. ± stimulus)

Sample Preparation

Instrumentation

Instrument
PBI Barocycler® NEP 2320 PCT SPS
Precellys Homogenizer
Tecan freedom EVO 100 Liquid handling system

Services

  • Protein digest (In solution, in gel, FASP, pressure-assisted)
  • SDS-PAGE
  • Isoelectric focusing (IEF)
  • Extraction from very small sample amounts
  • Metabolomics and proteomics of the identical sample (small sample amounts, e.g. biopsies)
  • Processing of formalin-fixed, paraffin-embedded (FFPE) samples
  • Processing of complex sample material, e.g. tissue samples, plasma (metabolomics), CSF, tear samples, urine