Euler Lab

Ophthalmic Research

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Methodology

We established a comprehensive method catalogue for optical measurements of light driven population activity throughout the retina's entire vertical pathway based on synthetic and genetically encoded calcium sensors.

Our cornerstone technique is two-photon microscopy, which enables us to excite fluorescent probes within the tissue using infrared laser light. This avoids bleaching of the light-sensitive photoreceptor pigment in and therefore allows recording of optical activity in intact retinal tissue while simultaneously stimulating with light patterns. We complement our optical approach by single cell electrophysiology.

Using viral transfection strategies we are currently complementing our methodological array to also include amacrine and horizontal cells. In combination with microelectrode- and anatomical techniques we unravelled strategies of visual information processing by microcircuits throughout the retina, ranging from dendritic and synaptic processing to the analysis of population activity.