Job Description: AREA III
ER (Postdoc)
Temporally and spatially regulated gene expression is a key factor for
cellular differentiation and basic molecular function of specific cell types.
The project aims to characterize the mechanisms and factors that rule and
regulate cell type specific gene expression in the retina. In the end cell
type specific promoters and means to regulate transcription are important
tools for the development of specific gene-based therapies. Project work
includes:
i) mapping of transcription initiation and termination sites,
ii) cloning of reporter gene constructs and quantitative expression analysis
in retinal cell lines,
iii) identification of transcription factor binding sites,
iv) characterization of basic gene transcription networks, and
v) identification of compounds that modulate gene transcription.
Methods applied: in silico database analysis, transcript
mapping, reporter gene constructs, reporter gene assays, EMSA, trans-activation
assays.
Candidate profile: Ph.D. or equivalent in Molecular Genetics,
Human Genetics or Biochemistry. We expect a candidate with much enthusiasm
and motivation in academic research. Practical knowledge in transcript mapping,
reporter gene cloning & analysis, quantitative expression analysis and development
of cell-based expression assays is advantageous.
The Molecular Genetics Laboratory at the University Eye Hospital
Tübingen is one of the leading centers for studies on hereditary retinal disorders
and the molecular biology of the retina in Europe. It offers excellent training
opportunities in diverse aspects of retinal genetics applying modern technologies
in a stimulating research atmosphere.
Applications including a CV, a list of publications and two letters of reference
should be sent to:
Dr. Bernd Wissinger
Molecular Genetics Laboratory
University Eye Hospital
Auf der Morgenstelle 15
D-72076 Tuebingen
Germany
E-mail: wissinger@uni-tuebingen.de
ESR (PhD Student)
Temporally and spatially regulated gene expression is a key factor for cellular differentiation
and basic molecular function of specific cell types. The project aims to characterize the mechanisms
and factors that rule and regulate cell type specific gene expression in the retina. In the end cell
type specific promoters and means to regulate transcription are important tools for the development of
specific gene-based therapies.
Project work includes:
i) mapping of transcription initiation and termination sites,
ii) cloning of reporter gene constructs and quantitative expression analysis in retinal cell lines,
iii) identification of transcription factor binding sites,
iv) characterization of basic gene transcription networks, and
v) identification of compounds that modulate gene transcription.
Methods applied: in silico database analysis, transcript mapping, reporter gene constructs, reporter gene assays, EMSA, trans-activation assays.
Candidate profile: M.S. or equivalent in Molecular Genetics, Human Genetics
or Biochemistry. We expect a candidate with much enthusiasm and motivation in academic research.
Some practical knowledge in biocomputing, molecular biology and/or molecular genetics is advantageous.
Ph.D. degree may be either applied for at the Faculty of Biology of the Eberhard-Karls-University Tübingen,
Germany (depending on the approval of prior degrees) or at a local university adhering to the regulations
for external theses.
The Molecular Genetics Laboratory at the University Eye Hospital
Tübingen is one of the leading centers for studies on hereditary retinal disorders
and the molecular biology of the retina in Europe. It offers excellent training
opportunities in diverse aspects of retinal genetics applying modern technologies
in a stimulating research atmosphere.
Applications including a CV, a list of publications and two letters of reference
should be sent to:
Dr. Bernd Wissinger
Molecular Genetics Laboratory
University Eye Hospital
Auf der Morgenstelle 15
D-72076 Tuebingen
Germany
E-mail: wissinger@uni-tuebingen.de