Schraermeyer Lab

Experimental Vitreoretinal Surgery

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Methodology

A broad range of experimental techniques is performed in our lab on experimental animals (mice, rats, rabbits, monkeys), ranging from surgery and drug treatment, in vivo diagnostics (OCT, SLO, ERG and VEP), and finally analysis of tissues (including from human donors) on histological, immunohistochemical and ultrastructural levels including immunogold and analytical electron microscopy.

A light microscopic composite image of choroidal neovascularisation (CNV) (magnification: x600). Toluidine blue-stained semithin (0.7 µm) sections from an AMD patient show thinned, distorted or ruptured (labelled by arrow heads) Bruch’s membrane (Bm) underlying exudates in the retina. Note the extensive degeneration of the neurosensory retina. Chor corresponds to choroid.

Transmission electron microscopy micrograph of an AMD patient showing a druse (Dr) located in the Bruch’s membrane (Bm).

Light microscopy of a monkey eye (retina-RPE-choroid complex). ONL = outer nuclear layer; PR IS = photoreceptor inner segments; PR OS = photoreceptor outer segments; RPE = retinal pigment epithelium; ChC = choriocapillaris; Ch = choroid; V = choroidal vessels; M = melanocyte.

Transmission electron microscopy micrograph of a monkey eye showing two cones (C) and two rods (R) outer segments interacting with the microvilli (Mv) of the RPE.

Transmission electron microscopy micrograph of a monkey retina showing rod inner segment (R IS), cilium (Cil) and rod outer segment (R OS).

Transmission electron microscopy micrograph of a monkey retina showing one cone (C) and three rods (R).