Institute for Ophthalmic Research

Institut für Augenheilkunde

Home » The Institute » Vacancies & Opportunities

04-05-2017

Master student with interest in molecular and cell biology

The core facility for Medical Bioanalytics in the Institute for Ophthalmic Research is looking for a highly motivated Master student with interest in molecular and cell biology to achieve the

‘Functional isoform-specific analysis of TTC30A and TTC30B using CRISPR/Cas9-mediated genomic editing.’

Project description: 

The connecting cilium (CC) in retinal photoreceptors represents a specialized primary cilium, separating the light sensitive outer from the inner segment. The CC is essential for outer segment assembly and inter compartment signaling transduction. Transport of cargo along the CC is mediated by the intraflagellar transport (IFT) machinery which is essential to move structural and signaling components. In our lab we use different cell lines harboring primary cilia in culture as simple ‘cilia and CC model’ to investigate the molecular function of different ciliary proteins. In preliminary experiments, we described a distinct ciliary phenotype in siRNA treated cells targeting TTC30A or B. To prove these results and analyze the cilia dependent function of TTC30A/B in more detail, stable knock-out cells using CRISPR/Cas9 mediated knock-out will be generated. Two major steps will drive the Master thesis: 1) Generation of TTC30A/B knock-out (transfection of cells, resistance dependent selection, genomic DNA isolation, PCR). 2) Analysis and description of knock-out and control cells using immunostaining, migration and proliferation assays, and analysis of protein interaction (affinity purification, Western Blot, mass spectrometry).

We offer:

The Institute for Ophthalmic Research (www.eye-tuebingen.de/ueffinglab/) and the core facility for Medical Bioanalytics are an integral part of the Centre for Ophthalmology as well as of the Quantitative Biology Center at the University of Tübingen (www.qbic.uni-tuebingen.de). We run a state-of-the-art facility for mass spectrometric analysis of proteins and their complexes. Our focus is to elucidate how gene variants and mutations affect protein complexes and protein networks and thereby cause disease. We can offer to work in a team of highly motivated scientists, a stimulating atmosphere and access to advanced technical infrastructure.

Required qualifications:

We are looking for a motivated candidate with high interest in cell and molecular biology. The applicants should send a pdf file containing a CV and copy of final certificates to tina.beyer[at]uni-tuebingen.de.

Application deadline: Mai, the 31th 2017.

To top

25-04-2017

Postdoctoral Scientist in Protein Network Analysis for AMD

The lab of Prof. Marius Ueffing at the Institute for Ophthalmic Research, University of Tübingen, Germany, is looking for a

Postdoctoral Scientist in Protein Network Analysis for Age-related Macular Degeneration

The Ueffing lab is applying systems medicine approaches to retinal degeneration. The lab runs a state-of-the-art facility for mass spectrometric analysis of proteins and metabolites. Within the EYE-RISK consortium the group collaborates with research institutions, eye clinics and industry to investigate the molecular drivers and risks of Age-related macular degeneration (AMD), Europe’s most frequent cause for blindness. EYE-RISK applies new methods in genetics, biochemistry and bioinformatics to build a robust and validated model of AMD pathways and risks. For insight into our methods and approaches see Boldt et al. 2016, Körtvely et al. 2016, Sacco et al. 2014, Kiel et al. 2011, Kiel et al. 2017.

Your tasks:

  • assembling the map of protein complexes and interactions in AMD
  • validating computational simulations of disease pathways by proteomics and cell biology (cf. Boldt et al. Nat Comm. 2016 for methods)
  • analysing biochemical properties of proteins coded by AMD risk variants (cf. Körtvely et al. Hum Mol Genet. 2016 for methods)
  • validating risk algorithms by analysing patient samples

Your profile:

  • background in proteomics, biochemistry or molecular biology
  • creativity & team spirit
  • experience in vision science, neurobiology, molecular genetics or protein science
  • talents in method development
  • decent publication record

We offer an environment for advanced research and close interaction with scientists in the lab and associated laboratories. You will work - in the systems medicine consortium EYE-RISK including leading European investigators, - in advanced analysis of the largest AMD patient cohorts in Europe, - within one of the leading Centers for Ophthalmology in Europe, - in a large and diverse lab firmly embedded into the neuroscience community on campus, - using the full spectrum of –omics technologies including SRM, phosphoproteomics, lipidomics, metabolomics, transcriptomics, RNAseq, and you can receive a competitive salary (according to your experience and within the legal framework of employment laws).

The position is available from the earliest date possible, initially limited to the duration of the EYE-RISK project until 30.04.2019, with plans for a further extension.

We offer remuneration in accordance with TV-L (collective wage agreement for the Public Service of the German Federal state) in addition to all the customary benefits granted to employees working in Public services. Severely handicapped persons with equal qualifications are given preferential consideration. The University of Tübingen is anxious to increase its quota of female scientific staff, and herefore emphatically requests women to apply for this position. The Administration of the University Hospital is responsible for all employment matters. Personnel appointments will be made pursuant to the fundamental stipulations of the legal statutes for universities in Germany.

Please include your cover letter, CV and the names of three academic references into a single pdf file and send your application to ellen.kilger[at]uni-tuebingen.de.

To top

23-11-2016

PhD Position in The Experimental Retinal Prosthetics group

The Experimental Retinal Prosthetics group (Dr. Daniel Rathbun and Professor Eberhart Zrenner) at the Institute for Ophthalmic Research at the Eberhard-Karls-University of Tübingen are offering a PhD position.

To be filled as soon as possible, with funding for 2+1 years. We offer employment with a salary and social benefits based on the collective agreement for public service employees in the academic and science sector, TV-L. The Eberhard-Karls-University of Tübingen promotes gender equality and therefore particularly encourages female scientists to apply.

The successful candidate will explore characterizing ganglion cells in the mouse retina by applying fluorescence microscopy and electrophysiology, e.g. microelectrode array and patch clamp. The goal of the project is selective activation of ganglion cell types with electrical microstimulation.

Requirements:

  • Background in neuroscience
  • Background in statistics and data analysis
  • Experience with electrophysiology
  • Programming experience in Python, Matlab, R or similar

The application package is expected to contain:

  • A curriculum vitae
  • Two reference letters
  • The motivation for applying to this position

Interested applicants should contact Dr. Daniel Rathbun (Daniel.rathbun[at]uni-tuebingen.de) or Dr. Zohreh Hosseinzadeh (zohreh.hosseinzadeh[at]uni-tuebingen.de).

To top