Institute for Ophthalmic Research

Institut für Augenheilkunde

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15-11-2017

PhD Positions on Dissection of the molecular function of ciliary proteins and their dys-function in disease.

The Ueffing lab at the Institute for Ophthalmic Research is looking for a highly motivated PhD student with high interest in molecular and cell biology to achieve the

Dissection of the molecular function of ciliary proteins and their dys-function in disease.

Project description:

The cilium protrudes from virtually any polarized cell within the human body. It is essential to see, hear, breath, digest, excrete, develop and reproduce. Therefore, dys-function of cilia causes a group of diseases called ciliopathies with varying severity and symptoms, including retinal degeneration. We use different cell lines harboring primary cilia in culture as a model to investigate the molecular function of different ciliary proteins.

This project aims at dissecting the function of specific ciliary proteins in health and disease by applying various, state of the art techniques. In large screens, we have identified several candidate proteins which have a potential role in cilia function (Boldt et al, Nature Communications 2016). CRISPR/Cas9 mediated knock-in strategies will be used to determine protein interactions at physiological levels which will reveal functional relevant protein complex assemblies. Further, knock-out models in combination with state of the art imaging techniques will be used to evaluate the role of these proteins in cellulo.

What we offer:

The Institute for Ophthalmic Research (www.eye-tuebingen.de/ueffinglab/) is an integral part of the Centre for Ophthalmology as well as the Quantitative Biology Center at the University of Tübingen (www.qbic.uni-tuebingen.de). We run a state-of-the-art facility for mass spectrometric analysis of proteins and their complexes. Our focus is to elucidate how gene variants and mutations affect protein complexes and protein networks and thereby cause disease. Further we are equipped with high-end imaging equipment, including a gSTED microscope. We can offer to work in a team of highly motivated scientists, a stimulating atmosphere and access to advanced technical infrastructure.

Required qualifications:

We are looking for a motivated candidate with a degree in life science (diploma/ masters) and a strong background in cell and molecular biology. Experience in cell culture, cloning and protein analytics is preferred. We offer salary according to the German salary scale TV-L for two years (beginning of project: February 1st, 2018), one-two - year extension is intended.

The applicants should send a pdf file containing a cover letter, cv, copy of final certificates and contact details of two academic referees to karsten.boldt[at]uni-tuebingen.de

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