Wissinger Lab

Molecular Genetics Laboratory

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Methodology

Molecular genetics: PCR, Sanger sequencing, Pyrosequencing, Next generation sequencing, High resolution melting technology and qPCR (Light cycler technology) and Taqman assays, linkage analysis and homozygosity mapping, gene copy number analysis (ie. CGH - comparative genomic hybridization, qPCR), associations studies

Molecular biology: Eukaryotic cell culture and bacterial culture, cloning, heterologous expression of recombinant proteins, patch clamp, enzymatic assays, Western blot, immunohistochemistry, yeast-two-hybrid analysis