Clark Lab

Molecular mechanisms driving age-related macular degeneration

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Kirsten Bucher

Surname: Bucher
First name(s): Kirsten
Nationality: German
Present position and title: Postdoctoral research scientist; Dr. rer. nat.

Business address

Institute for Ophthalmic Research
Centre for Ophthalmology,
University of Tübingen
Elfriede-Aulhorn-Strasse 7
D-72076 Tübingen,

Phone: +49 7071 29 87893
E-mail: kirsten.bucher[at]

Professional Experience

Period Institution Position Discipline
Since 2019 Institute for Ophthalmic Research, Tübingen, Germany Postdoctoral research scientist Immune responses to retinal gene therapy
2016 - 2018

Department of Clinical Affairs; CRYOLIFE, Hechingen, Germany

Medical Writer Clinical Research
2010 - 2016 Institute of Pharmacology and Toxicology, Tübingen, Germany Postdoctoral research scientist Immunopharmacology                              

Academic Education

Year Degree University Field of study / Subjects
2005-2010 Dr. rer. nat. University of Tübingen Parasitology / Immunology
1992-1995 Staatsexamen University of Tübingen Biology and English
1987-1992     Studies of Biology;
Subjects: Zoology, Parasitology, Pharmacology, Microbiology

Professional Activities and Functions

Academic Teaching

Date Description
2010 - 2016 Seminar „General and systematic pharmacology and toxicology“ for medical students;
Topics: Antiviral drugs, Asthma, Anti-microbial chemotherapy

Past and Current Funding

Date Description
2022 Research grant from the PRO RETINA-Stiftung, Germany
2019 - 2021 DFG funded priority programme SPP 2127

Selected Publications

  1. Pfromm, J. K., Bonillo, M., Dauletbekov, D., Bucher, K.1 & Fischer, M. D., 2022. Plasmid-mediated gene transfer of Cas9 induces vector-related but not SpCas9-related immune responses in human retinal pigment epithelial cells. Sci Rep. 12, 13202. 1 corresponding author and equal last author
  2. Bucher, K.1, Rodríguez-Bocanegra, E.1, Fischer, M.D. Benefits and shortcomings of in vitro model systems in the development of retinal genetic therapies. (Accepted at Klin Monbl Augenheilkd. Jan 2022); 1 equal first author
  3. Bucher, K., Rodríguez-Bocanegra, E., Dauletbekov, D., Fischer, M.D., 2021. Immune responses to retinal gene therapy using adeno-associated viral vectors – Implications for treatment success and safety. Prog. Retin. Eye Res. 83, 100915.
  4. Rodríguez-Bocanegra, E., Wozar, F., Seitz, I.P., Reichel, F.F.L., Ochakovski, A., Bucher, K., Wilhelm, B., Bartz-Schmidt, K.U., Peters, T., Fischer, M.D., 2021. Longitudinal evaluation of hyper-reflective foci in the retina following subretinal delivery of adeno-associated virus in non-human primates. Transl. Vis. Sci. Technol. 10, 1–14.
  5. Beer-Hammer, S., Lee, S.C., Mauriac, S.A., Leiss, V., Groh, I.A.M., Novakovic, A., Piekorz, R.P., Bucher, K., Chen, C., Ni, K., Singer, W., Harasztosi, C., Schimmang, T., Zimmermann, U., Pfeffer, K., Birnbaumer, L., Forge, A., Montcouquiol, M., Knipper, M., Nürnberg, B., Rüttiger, L., 2018. Gα i Proteins are Indispensable for Hearing. Cell. Physiol. Biochem. 47, 1509–1532.
  6. Bucher, K., Schmitt, F., Mothes, B., Blumendeller, C., Schäll, D., Piekorz, R., Hirsch, E., Nürnberg, B., Beer-Hammer, S., 2017. Deficiency of PI3-Kinase catalytic isoforms p110γ and p110δ in mice enhances the IL-17/G-CSF axis and induces neutrophilia. Cell Commun. Signal. 15, 1–1
  7. Mothes, B.1, Bucher, K.1, Ammon-Treiber, S., Schwab, M., Piekorz, R.P., Hirsch, E., Nürnberg, B., Beer-Hammer, S., 201 P110γ/δ double-deficiency induces eosinophilia and ige production but protects from ova-induced airway inflammation. PLoS One 11, 1–19. ; 1 equal first author
  8. Tsvetkov D., Shymanets A., Huang Y., Bucher K., Piekorz R., Hirsch E., Beer-Hammer S., Harteneck C., Gollasch M., B. Nürnberg B., 2016. Better Understanding of Phosphoinositide 3-Kinase (PI3K) Pathways in Vasculature: Towards Precision Therapy Targeting Angiogenesis and Tumor Blood Supply. Biochemistry (Moscow) 81, 691-699.
  9. Carevic, M., Öz, H., Fuchs, K., Laval, J., Schroth, C., Frey, N., Hector, A., Bilich, T., Haug, M., Schmidt, A., Autenrieth, S.E., Bucher, K., Beer-Hammer, S., Gaggar, A., Kneilling, M., Benarafa, C., Gao, J.L., Murphy, P.M., Schwarz, S., Moepps, B., Hartl, D., 2016. CXCR1 Regulates Pulmonary Anti- Pseudomonas Host Defense. J. Innate Immun. 8, 362–373.
  10. Bucher, K., Schmitt, F., Autenrieth, S.E., Dillmann, I., Nürnberg, B., Schenke-Layland, K., Beer-Hammer, S., 2015. Fluorescent Ly6G antibodies determine macrophage phagocytosis of neutrophils and alter the retrieval of neutrophils in mice. J. Leukoc. Biol. 98, 365–372.
  11. Schmitt, F., Schäll, D., Bucher, K., Schindler, T.I., Hector, A., Biedermann, T., Zemlin, M., Hartl, D., Beer-Hammer, S., 2015. SLy2 controls the antibody response to pneumococcal vaccine through an IL-5Rα-dependent mechanism in B-1 cells. Eur. J. Immunol. 45, 60–70.
  12. Shymanets, A., Prajwal, Bucher, K., Beer-Hammer, S., Harteneck, C., Nürnberg, B., 2013. P87 and p101 subunits are distinct regulators determining class I B phosphoinositide 3-kinase (PI3K) specificity. J. Biol. Chem. 288, 31059–31068.
  13. Bucher, K., Dietz, K., Lackner, P., Pasche, B., Fendel, R., Mordmüller, B., Ben-Smith, A., Hoffmann, W.H., 2011. Schistosoma co-infection protects against brain pathology but does not prevent severe disease and death in a murine model of cerebral malaria. Int. J. Parasitol. 41, 21–31.
  14. Bahamontes-Rosa N., Bucher K., Held J., Robin A., Hoffmann W. H., Flitsch S. L., Kremsner P. G., Kun J. F., 2009. In vivo anti-malarial effect of the β-amino alcohol 1t on Plasmodium berghei. Parasitology Research 104, 1459-1464.