Wissinger Lab

Molecular Genetics Laboratory

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Molecular genetics

PCR; Sanger sequencing; Pyrosequencing; Next generation sequencing; High resolution melting technology and qPCR (Light cycler technology) and Taqman assays; Linkage analysis and Homozygosity mapping; Gene copy number analysis (ie. CGH - comparative genomic hybridization, qPCR); RNA-Seq; Association studies; TALEN and CRISPR/Cas9 technology

Molecular biology

Eukaryotic Cell culture and Bacterial culture; Cloning; Heterologous expression of recombinant proteins; Heterologous splice assays, Enzymatic assays; Western blot; Immunohistochemistry; In situ hybridization; Yeast-two-hybrid analysis; Zebrafish manipulation, generation and characterization.